Charter student completes research in Seyfferth’s lab

February 19, 2014 under CANR News

charter student completes research in Angelia Seyfferth's labWhen Rohith Venkataraman, a junior who attends the Charter School of Wilmington, decided to search for research being conducted by University of Delaware professors, he did not know what he would find. By chance, he came across Angelia Seyfferth’s research and sent her an e-mail asking if he could help out in her lab.

Now, about a year since that e-mail was sent, not only has Venkataraman completed his research with Seyfferth, assistant professor in the Department of Plant and Soil Sciences, and Gang Li, a postdoctoral researcher in Seyfferth’s lab, but that research helped him place first in the plant science section of his school’s science fair. He will now be presenting his research project at the 19th annual Delaware Technical Community College Science Expo in February.

“It’s safe to say that email was a great decision,” said Venkataraman. “Dr. Seyfferth has been encouraging and helpful right from the get go and I have had a wonderful research experience under her guidance and Dr. Li’s tutelage.”

The research began last summer when Venkataraman worked with Seyfferth and Li on a plant science project dealing with the arsenic uptake mechanism in rice plants. Venkataraman said that Seyfferth’s previous work suggested that one can decrease the amount of arsenic assimilated by a plant if one adds silicic acid to the growth media of rice plants.

“This promising research could lead to new ways of growing rice in areas with high arsenic contents in the soil,” said Venkataraman. “As a means to confirm literature and test our own variables, we designed and grew plants over a period of 3 weeks, 6 weeks, and 9 weeks. The plants were then flash frozen and stored in a freezer for further RNA analysis of oxidative stress genes and total arsenic content analysis.”

Venkataraman said that the procedure for extracting RNA and analysis was “quite complex. There were many steps each requiring careful attention to details. The procedure required adding small quantities of many solutions and centrifuging the tissue numerous times.”

As for his favorite part of the project, Venkataraman explained that he enjoyed assisting Li with changing the nutrient solutions for the plants and assisting with the liquid nitrogen flash freezing.

“The attributes of chemicals and their properties have always amazed me,” said Venkataraman. “I have always looked forward to Dr. Seyfferth’s insight on scientific aspects of the research, which she has enthusiastically shared with me. Dr. Li has guided and explained about various processes that we were working on. Both of them have expanded my horizon of knowledge and made my experience at the lab and research something that I have looked forward to.”

Seyfferth said that she was “impressed by Rohith’s questions and scientific inquisitiveness, he is a highly motivated young student.” Seyfferth added that she is “eager to continue providing research opportunities for high school students across Delaware.”

As for his plans for after high school, Venkataraman said that he is not sure what he wants to do yet but that he is exploring studying molecular biology along with a pre-med track.

Article by Adam Thomas

Photo by Danielle Quigley

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